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Ethics declarations Ethics approval and consent to participate Protocols of animal experiments were permitted by Institutional Animal Care and Use Committee (IACUC), Ocean University of China

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The membranes were then washed several times with water and TBS (PBS, 0.05% Tween-20), a 1 :5000 dilution of horseradish-peroxidase conjugated goat anti-rabbit antiserum was added, and the membranes further incubated for 1 h at room temperature