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After infection, infected cells were fixed with Histofix 4% (Carl Roth) for 15 min at RT

Studies have shown that when the cystine content in the TME increases, the dependence of tumor cells on glutamine metabolism can be reduced through cystine/glutamate antiporters

The CDC states that unopened vials in this category should be discarded according to the manufacturers expiration date

At room temperature storage, melatonin treatment reduced the relative membrane-leakage rate and inhibited the generation of superoxide radicals (O 2 ), ethylene synthesis, hydrogen peroxide (H 2 O 2 ) and malondialdehyde (MDA), resulting in prolonged freshness and a reduction in browning and degradation of chlorophyll/pigments in fruits and vegetables (176)