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The ER agonist (1 M), ER antagonist (10 M) and estriol (1 M) were dissolved in DMSO and then diluted to the desired concentration with the culture medium for the experiments

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Mechanism of Action The mechanism of action of GHK-Cu relies on its ability to extensively modulate gene expression

Movement across the BBB occurs via transmembrane efflux pumps (Abbott et al., 2010) that transport molecules through endocytosis or through pores, carrier-mediated transport systems, or through direct permeation through the plasma membrane via lipophilic molecules (Banks and Kastin, 1992)