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Subsequently, the ATP6AP1L coding sequence was amplified from the cDNA of T47D cells and inserted into the FastDigest KpnI/FastDigest XbaI (Thermo Fisher) linearized pcDNA3.1-GST DAF and pcDNA3.1-GST CEAM7 vectors using T4 DNA ligase (EL0011, Thermo Fisher)

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The second-generation anti-androgen drugs such as enzalutamide or abiraterone in standard treatment of advanced prostate cancer combined with ferroptosis activator can further inhibit tumor proliferation [17]

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