glutathione dosage per day injection Compounded What is the typical dosage

Preclinical studies have administered 5-Amino-1MQ in rodent dietary models to observe whole-body metabolic parameters, including body composition, energy expenditure, and plasma metabolite profiles

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Lysate was applied on the column and contaminants were removed using Wash buffer 1 (50 mM Tris, pH 7.5, 150 mM NaCl, 20 mM imidazole and 2 mM TCEP) and Wash buffer 2 (50 mM Tris, pH 7.5, 1 M NaCl, 20 mM imidazole and 2 mM TCEP)

Could someone answer either of these questions for me.both the sudden drop in efficacy ,if there is one,and the later observation