HA-Ubiquitin-propargylamide (human) (rec.) (HA) HY-50896 Many affinity-based methods require harsh

Many affinity-based methods require harsh elution condition that may affect the activity of isolated proteasomes and limit certain downstream applications

For instance

FCCP is an uncoupler of oxidative phosphorylation (OXPHOS) in mitochondria

Due to the use of protein extraction filter cartridges

HA-Ubiquitin-propargylamide (human) (rec.) (HA) HY-50896 Many affinity-based methods require harshUbiquitin is a small (8. 5kDa) regulatory protein that has been found in almost all tissues of eukaryotic organisms. The addition of ubiquitin to a substrate protein is called ubiquitination or ubiquitylation. Ubiquitination can affect proteins in many ways: it can signal for their degradation via the proteasome, alter their cellular location, affect their activity and promote or prevent protein interactions. Removal of ubiquitin from a substrate