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JAMA 251 , 22232230 (1984)

The 10mg format provides sufficient material for multiple experimental runs without frequent reordering, aligns with quantities referenced in the majority of published laboratory research, offers better cost efficiency per milligram for established programmes, and maintains stability in lyophilised storage at -20C across a typical research project

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The main advantage of omitting trypsin use from MS sample preparation is that peptide ends are likely to arise from endogenous protease cleavage sites, thus increasing sensitivity for identification of relevant peptide proteoforms 19