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Most were in much better condition than that first gentleman I had met, but they had more variations in the symptoms of their disease, including pediatric patients, mostly with liver disease, and older patients with neurological and mental health complications

Next, the polymerase was diluted 1:80 with amplifying solution, and, finally, the amplifying solution was diluted 1:5 with ultrapure water

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Immunofluorescence images of the F-actin cytoskeleton, stained with Phalloidin (cyan), highlighted a gradual change at the cellular level and in the structural organisation of the hiPSC colonies (Fig