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HSA was immobilized to the surface by pre-activation (1.0 EDC, 1.0 M NHS in PBS, pH 7.4, 7 min) followed by injection of HSA to flow cell 1 (30 g ml 1 in 10 mM NaOAc buffer, pH 5.0, twice for 7 min)
Author contributions PS, SG, RK, BW, SK, CD, and JL-B designed the study, enrolled patients, searched the published work, analyzed and interpreted data, wrote the report, reviewed the manuscript, and approved the final draft
It was noted that IGF-1 LR3 might potentially be 1.5 to 2 times as anabolic as IGF-I in inducing weight gain, increasing visceral organ weights, and possibly enhancing feed use efficiency under continuous delivery conditions
doi: 10.3389/fnut.2023.1015046 Received 09 August 2022 Accepted 16 February 2023 Published 06 March 2023 Volume 10 - 2023 Edited by Domenico Sergi, University of Ferrara, Italy Reviewed by Tianlin Gao, Qingdao University, China
