Na + -dependent glutathione transporters are found on the luminal side of the capillary endothelial cells of the brain, kidneys, and small intestine while its presence on the luminal side of the capillary endothelial cells of other organs is very minimal
After incubation, the protein solution was analysed by gel filtration chromatography on a HiLoad 16/60 Superdex 200 prep grade column (GE Healthcare), which was connected to an KTAFPLC system (GE Healthcare)
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The sulfhydryl group (-SH) on glutathione's cysteine residue binds directly to heavy metals including mercury, lead, cadmium, and arsenic, forming glutathione-metal conjugates that can be excreted through bile and ultimately eliminated from the body