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The reaction is catalyzed by the enzyme fumarate reductase

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Following the injection, in vivo imaging was conducted using an IVIS Spectrum (PerkinElmer) at various time points: 1 h, 2 h, 4 h, 6 h, 8 h, 12 h, 24 h, and 48 h post-injection, utilizing an excitation wavelength of 780 nm and a fluorescence emission wavelength of 840 nm

*Correspondence: Giulia Guerriero, Department of Biology, Comparative Endocrinology Lab, Universit degli Studi di Napoli Federico II, Via Mezzocannone, 8, Naples 80134, Italy e-mail: [email protected] This article was submitted to Experimental Endocrinology, a section of the journal Frontiers in Endocrinology