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Manipulation of urine samples

The assay plate was sealed and incubated at room temperature with gentle shaking for 30 min (300 rpm) while protected from light

J Neurosci Res 80:655666 Woehrling EK, Hill EJ, Coleman MD (2007) Development of a neurotoxicity test-system, using human post-mitotic, astrocytic and neuronal cell lines in co-culture

All commercial syntheses are based on the concept of a three-component, one-pot reaction of triamino-pyrimidinone with a three-carbon compound of variable structure (e.g., halogen derivatives of propanal, propanone, and propane) and p -aminobenzoyl-L-glutamic acid to yield folic acid