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Immunoblot of indicated proteins in HEK-293T cells expressing 3xFLAG- SLC25A39-P2A-RFP treated with BSO (1 mM) and erastin (5 M) for 48 h (bottom)

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Alternatively the continuous gradient might be produced by diffusion from a discontinuous gradient using 2.5 ml each of 1.040 g/ml (containing cells), 1.075g/ml, 1.110 g/ml and 1.145 g/ml (equivalent to approx 6%, 13%, 20% and 27% (w/v) iodixanol)