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The barrier is characterized by an enrichment of interendothelial tight junction proteins, several luminal and abluminal transporters, and luminal efflux transporters, which together maintain separation of the blood and brain and tightly regulate molecular trafficking between the blood and brain and vice versa 20

The precipitate is then dialyzed against PBS (pH 7.2) and triphosphate buffer (pH 8.2), followed by further purification and analysis using gel electrophoresis and chromatography

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