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To annotate the library, the Homo sapiens UniProt one protein sequence per gene database (UP0000005640_9606, downloaded 7 April 2021) was used, with deep learning-based spectra and RT prediction enabled

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The cell pellet was resuspended to a 40% final hematocrit with inactive AB human serum (Sigma-Aldrich) and the blood solution (as described in section 2.6) was offered to the mosquitoes for a maximum of 120 minutes