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Data availability The expression datasets analyzed for this study can be downloaded from the TCGA database ( The correlation between drug sensitivity and mRNA expression was analyzed using the Gene Set Cancer Analysis (GSCA) database ( The relationships between sorafenib and four ferroptosis-related genes in RCC were analyzed using the Genomics of Drug Sensitivity in Cancer (GDSC) database ( All data generated or analyzed during this study are included in this article

Statistical analysis The statistical methods used in this study were carefully selected to ensure robust and reliable results

Floh et al., 1997)

For the remaining candidate genes tatC , manC , and mobA , along with the waa locus responsible for biosynthesis of the LPS inner and outer corethere were no immediate red flags regarding the specificity of their phenotypes based on the initial transposon mutagenesis screen