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The intraluminal suture was inserted through a small puncture opening in the ECA and advanced into the ICA by approximately 18 mm (adjusted for rat weight) until a slight resistance was felt

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At day 21, four rabbits in the TCC group and three in the zinc oxide group achieved complete coverage with granulation tissue and epithelialization, with mean healing times of 18.4 and 21.5 days, respectively

Administering antioxidants prior to or alongside gonadotoxic treatments may mitigate ROS induced damage to spermatogenic cells, thereby preserving endogenous sperm production