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Determination of serum biochemical parameters Serum BUN (mg/dl), creatinine(mg/dl), uric acid(mg/dl), Ca (mg/dl), P(mg/dl) and total protein (g/dl) were measured at the end of the study by an enzymatic colorimetric assays with a DIRUI (CS-T240, China) auto-clinical chemistry-analyzer and commercial diagnostic kit Biosystem company, Spain

Shaking instead of rolling: Similarly problematic to rapid injection

Our study demonstrated that methylated UBE2m increases the neddylation level and protein stability of NEDD4

Faecalibacterium Prausnitzii -Derived Microbial Anti-Inflammatory Molecule Regulates Intestinal Integrity in Diabetes Mellitus Mice Via Modulating Tight Junction Protein Expression