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Description

ERS activating unfolded protein reaction (UPR) pathways is recognized by 3 sensors in endoplasmic reticulum (ER) membrane: protein kinase RNA-like endoplasmic reticulum kinase (PERK), inositol-requiring protein 1 (IRE1), and cyclic AMP-dependent transcription factor 6 (ATF6)

The membranes were blocked using 2% BSA in Tris-buffered saline, 0.05% TWEEN (TBS-T) for 60min and incubated overnight at 4C with the indicated primary antibodies