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We further carried out 60-ns MD simulations with 3 replicas for PV2-C23pAcF and PV2-C96pAcF variants to understand the effect of pAcF incorporation on protein conformation, and the RMSD, RMSF, radius of gyration, the existence of hydrogen bonds and salt bridges were analyzed (Figs
How Serial Dilution Works Reconstitute the peptide at a high concentration (e.g., 2 mg/mL) Take an aliquot and dilute 1:1 with bacteriostatic water 1 mg/mL Take an aliquot from the 1 mg/mL solution and dilute 1:1 0.5 mg/mL Continue as needed for the research protocol Important : Each dilution step must use sterile equipment and fresh bacteriostatic water
Among this latter group of medications, TMA lyase inhibitors have shown a significant ability to decrease serum TMAO without possessing many theoretical downsides
Reconstituted solution should be refrigerated at 28C and used within 28 days or aliquoted and frozen
