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Chromatin was extracted by addition of 1 ml of NUN2 buffer containing 20 mM HEPES (pH = 7.6), 7.5 mM MgCl 2 , 0.2 mM EGTA, 300 mM NaCl, 1 M urea and 1 % NP-40, 1x protease and 1x phosphatase inhibitors and rotated at 4 C for 10 min

The characteristics of polymeric nanocomposites depend on several parameters: the synthesis method, location and type of nanofillers, the degree of interface interaction, the concentration and nature of nanofillers, the type of developed transition layer, size and morphology of nanomaterials, and overall system architecture

Furthermore, human Grx1 reacts twenty times faster with GSH than with L-cysteinylglycine or L-cysteine, revealing that the preference for GSH also depends on its -glutamyl moiety 19

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