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Regulation of survivin expression through Bcr-Abl/MAPK cascade: Survivin as a therapeutic target in STI571 resistant CML cells

Protocol 4: nasal spray approach Goal: Needle-free administration with acceptable bioavailability trade-off Duration: 20 days active, then 4-6 months off Schedule: Days 1-20: 10-15 mg epitalon intranasally, divided into 2-3 daily doses Days 21-180: Off cycle Repeat 1-2 times per year Formulation: Reconstitute in saline with 3 percent DMSO for improved nasal absorption

B.et al (2022)

After TMB substrate solution is added, only those wells that contain Human IGFBP3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color