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Selection of diffusion medium: The receiving medium placed in the diffusion cells must be one which mimics the physiological condition of the skin

Then constructing cDNA library and sequencing were carried out according to the procedures by Novogene Co., Ltd

TB-500 TB-500 is a synthetic peptide fragment of Thymosin Beta-4, a naturally occurring protein found in almost all human and animal cells

Dynamics and line broadening: rapid conformational fluctuations within IDPs/IDRs, particularly on the millisecond to microsecond timescale, can lead to significant line broadening in NMR spectra, sometimes preventing direct studies of partially folded intermediates like molten globules