Each variable was scored for each sample slide, and we used a common scoring system where [0] = no alteration, [1] = mild changes, [2] = moderate changes, and [3] = severe changes ( 2.8 Immunohistochemical analysis Paraffin sections from different groups of liver tissues from rats were stained by immunohistochemistry (IHC) according to the manufacturing protocol using Anti-caspase 3 antibody (ab4051), at dilution 1:1,000, Anti-Bax antibody (ab32503), at dilution 1:250 and Anti-Bcl-2 antibody (ab182858), at dilution 1:500, (abcam, Cambridge, United Kingdom)
Samms RJ, Christe ME, Collins KA, et al
4a, bottom and Supplementary Data 2), suggesting that MZB rely on glycolysis rather than oxidative metabolism at steady-state
Then, the tissue was homogenized in 5 ml/gm cold buffer, which consisted of 50 mM tris-HCl, pH 7.5, 5 mM EDTA, 1 nM DTT