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As an illustration, investigations into the impact of 1-methyl-4-phenylpyridinium (MPP+), known for its neurotoxic effects mediated by various mechanisms, including complex I (CI) inhibition, demonstrated elevated glutathione (GSH) levels coupled with the upregulation of ATF-4 and the transsulfuration enzymes CTH and CBS

Hadamard Encoding and Reconstructions of MEGA-edited Spectroscopy HERMES, which was used in this study), allows us to differentiate GSH from other overlapping signals, providing better specificity and accuracy of the measured metabolite levels, and thus increased reliability of our results

The enzymatic activity of recombinant GpoA was examined in vitro to elucidate its biochemical properties

Pathway enrichment analysis was performed using the web-based tool EnrichR [39] ( leveraging both Reactome and Gene Ontology annotations