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OCT4, a pluripotency marker, was also stained for, but showed negative expression (data not shown), indicating a highly efficient induction of DE cells

The GST activity is determined by measuring the rate of produced conjugation between reduced glutathione and CDNB, which is proportional to the increase in absorbance at 340nm over time (OD 340nm /min)

reported that DHODH (Dihydroorotate dehydrogenase) coordinates with GPX4 by reducing ubiquitin formation in cancer cells, blocking the action of ferroptosis in mitochondrial intima [52], DHODH inhibitors induce ferroptosis and significantly inhibit tumor growth in solid tumors with low GPX4 expression, and the combination of ferroptosis inducer sulfasalazine and DHODH inhibitors has a good therapeutic effect in solid tumors with high GPX4 expression