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Commonly a time period of up to 5 h post-ingestion is considered to represent the small intestine, whilst between 5 to 24 h represents the colon [60, 67]

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The Stromal, Immune and ESTIMATE scores of LSG samples were calculated using the ESTIMATE R package ( The single-sample gene set enrichment analysis (ssGSEA) (31) and CIBERSORT ( algorithms were used to estimate the proportion of innate and adaptive immune cell types in the LSGs of patients with pSS