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Tu et al., 2019

ECG Recording ECGs were recorded continuously in deeply anesthetized rats for all three main leads, by positioning stainless steel electrodes on all four limbs using an ECG monitor with a 2090 programmer (Medtronic, United States) connected to a Waverunner LT342 digital oscilloscope (LeCroy, United States) at 30 min ligation time

However, avoid leaving packages in hot mailboxes, trucks, or outdoors for extended periods

8 weeks (11 vials): 33 mL 4 10 mL bottles 12 weeks (16 vials): 48 mL 5 10 mL bottles 16 weeks (22 vials): 66 mL 7 10 mL bottles Alcohol Swabs: One for the vial stopper + one for the injection site each day