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Dark Fired, sun-cured, and plenty of other methods are utilized for Virginia tobacco, which we will explore further in future posts

While the subcellular localisation is certainly the central finding of this work, the overexpression of Nt TPC1A allows to extract information on the cellular functions of this channel adding to previous work on NTTPC1 that was either done in patch-clamp models (perfect for biophysical aspects, missing out on cell biology and physiology) or in mutants of Arabidopsis (perfect for developmental aspects, missing out on cell biology and physiology)

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