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The tissue samples were homogenized in PBS (pH 7.4) containing 0.1 mmol/L butylated hydroxytoluene (BHT) (TissueRuptor, Qiagen, United States) and sonicated for 30 s in an ice bath (Ultrasonic bath, Branson, United States)

This either suggests a genuinely pleiotropic mechanism or that some of the reported effects are artifacts of study design or publication concentration in a single research group

Bee updated: current knowledge on bee venom and bee envenoming therapy