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Despite the superior performance of this fluidics approach, it is not a straightforward method in terms of the development of the experimental setup, which could limit its wide applicability and commercial uptake

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The permeability of the leak pathway can be better assessed ex vivo by measuring the flux of molecules from the luminal to the basolateral side such as EDTA, mannitol, sucrose, inulin and polyethylene glycols (PEG) or dextran molecules of variable sizes (ranging from 4 up to 20 KDa)