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Samples were permeabilized with 0.4 M glycine and 0.3% Triton X-100, and nonspecific binding was blocked with 2% normal swine serum (DAKO, Glostrup, Denmark)

Earaches and ear pain: causes, treatment, and prevention Earaches and ear pain: causes, treatment, and prevention 2 minutes Updated 3 April 2024 Reviewed 19 January 2025 Children are often more at risk of getting earaches, but earache in adults is not uncommon either

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