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259 , F863F866 (1990)

Targeted metabolomics data collection For each sample, 20 L CSF or 5 L plasma was extracted by brief sonication in 200 L or 300 L extraction buffer, respectively (80% methanol, 25 mM ammonium acetate, 2.5 mM sodium ascorbate in LCMSgrade water), supplemented with isotopically labeled amino acids (Cambridge Isotope Laboratories, MSK-A2-1.2, Tewksbury, MA USA), aminopterin, and reduced glutathione (CNLM-6245-10)

This is a key difference from GLP-1 medications like Semaglutide, whose primary mechanism for weight loss is significantly reducing hunger signals