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Then came Dead & Company, which has restarted the Deadhead engine in a way that few could have anticipated: filling stadiums with fans who weren't born when the Grateful Dead disbanded while also pleasing older Heads who had every reason to be skeptical

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The proteins were extracted and transferred to a nitrocellulose membrane through via three different methods: Semi- dry transfer machine (Trans-Blot SD Semi-Dry Transfer Cell, Bio-Rad), Wet transfer machine (Wet/Tank Blotting Systems, Bio-Rad), and Turbo-transfer machine (Trans-Blot Turbo Transfer Starter System, Bio-Rad)

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