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A single strength (1X) Tris-Acetate-EDTA (TAE) buffer contains 40mM Tris-Acetate and 1mM EDTA
isolation of WAT and brown adipose tissue (BAT) is technically challenging due to their high lipid and low protein contents
Kowalczyk-Quintas
Due to these limitations
Mouse IL-6 (Interleukin 6) ELISA Kit Pack Size:96T A single strength (1X) Tris-Acetate-EDTAThis kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IL 6 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with IL 6 bound to precoated antibody. Wash unbound components and add HRP Streptavidin Conjugate (SABC). Wash
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