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You have reintroduced water, which means hydrolysis becomes an active threat, microbial contamination becomes possible, and the compound shelf life shrinks from years to weeks

Blood samples were immediately centrifuged, and plasma was transferred to pre-prepared tubes containing acetonitrile and an internal standard of Nle-YI-(6) aminohexanoic amide, 100 g/ml in isotonic saline

Label with date and refrigerate at 28 C (35.646.4 F) , protected from light

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