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In addition, chemical treatment (such as p-nitrobenzyl chloride, iodomethane) decreases GSH content in guard cells, in turn, increases the ROS accumulation significantly and thereby enhances guard cell sensitivity to ABA signaling (Okuma et al

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Electrons are then transferred from the oxidoreductase flavoenzyme to the appropriate electron carrier, either oxidized thioredoxin (Trx-S 2 ) or glutathione disulfide (GSSG) converting them to reduced thioredoxin (Trx-[SH] 2 ) or glutathione (GSH), respectively