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To measure the wound size (area), the images were analyzed using the ImageJ software and wound closure rates were quantified via equation 5: 2.8 Histological analysis To carry out histological analysis, the rats underwent euthanasia through intraperitoneal injection of ketamine (300 mg/kg) and xylazine (20 mg/kg) and wound tissues were completely excised on days 7, 14 and 21 post-treatment

doi: 10.1111/ene.12099 150 ChoSHJoACasaleTJeongSJHongSJChoJKet al

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All cell lines were cultured in RPMI 1640 media (Gibco) supplemented with 10% Fetal Bovine Serum (FBS) (Gibco), 1% L-Glutamine (Gibco) and 1% Penicillin/Streptomycin (Gibco)