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The slides were then rinsed with PBS buffer and mounted with a 1:1 (v/v) PBS/glycerol solution before being stored at 4 C overnight

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The numbers of such figures on sidewalks only started to decline in the 1900s as bye-laws stopped the creation of obstructions on streets

[65] It was also previously present in the Lapita culture, based on archaeological remains from Mussau dated to around 3,600 to 2,500 BP