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Chio, C
Shurygina IA, Prozorova GF, Trukhan IS, Korzhova SA, Dremina NN, Emel'yanov AI

Gene silencing of Si Orco and Si OBP5 through dsRNA injection (RNA interference) reduced gene transcription at 72 hours post-treatment and disrupted detection of 2,4,6-trimethylpyridine according to electroantennography assays ( Si OBP1 was highly expressed in the antennae of worker ants, and its protein was identified in the antennae proteome of males and workers ( Si OBP1 protein was predicted through an in silico approach and confirmed through experimental methods ( Si OBP1protein exhibited the highest affinity for -caryophyllene (0.1 g/L) and the lowest affinity for 2,5-dimethylhexane (0.1 g/L) ( Si OBP1 gene was silenced via RNAi ( On the other hand, Si OBP2 and Si OBP14 modulate workers foraging behavior ( Si OBP2 was expressed in the sensilla basiconica of the RIFA workers antennae and was involved in detecting the alarm pheromone 2,4,6-trimethylpyridine, while Si OBP14 was expressed in the antennae of workers and males ( Si OBP2 gene via RNAi disrupted OBP2 protein binding to the bait sausage odorants 3-mercapto-2-butanone and furfuryl mercaptan in accordance with electroantennography assays ( Si OBP14 gene silencing (RNAi) led to a shift from attraction to repellency towards 2-methyltetrahydrofuran-3-one, 2-methyl-3-furanthiol, 4,5-dimethylthiazole, and 2-methyl-3-methyl(thio)furan released by bait sausages for RIFA workers ( Si OBP2 and Si OBP14 gene repression significantly impaired the bait-sausage-searching efficiency of RIFA workers ( The general protein 9 (Gp-9) or Si OBP3 determines the social structure of RIFA colonies
