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Although this may be surprising, given that most marine life exists in near-shore, often estuarine conditions, where environmental fluctuations are common and define life activities, very few laboratory studies reflect such variable conditions ( 2 excreted, if animals are kept for extended periods of time requiring regular seawater changes using pH-adjusted clean seawater ( It is widely accepted that the choice of culture conditions for kept specimens represents a major challenge that potentially impacts results of any behavioral and physiological assay undertaken

doi: 10.1016/j.intimp.2018.12.030 20 FruhbeckGCatalanVRodriguezARamirezBBecerrilSSalvadorJet al